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Purified CD4 + T cells from HDs ( n = 3) and patients with acute ATL ( n = 5) were stimulated with anti-CD3 and anti-CD28 in presence of IL-2 for 4 hours at 37°C. The cells were harvested and subjected to scRNA-seq. ( A ) Schematic representation of the experimental setup for ( B to H ). Created in BioRender. S. Roy (2025) https://BioRender.com/9zdi679 . (B) Uniform Manifold Approximation and Projection (UMAP) plot showing all cells split by HD cells (left) and ATL cells (right). (C) Bar plot showing proportion of the cells in each cluster annotated by Azimuth for each HD and ATL sample. (D) UMAP plot showing distribution of T reg cells split by HD cells (left) and ATL cells (right). (E) Violin plot showing PRDM1 expression in T reg cells in HDs and patients with ATL. (F) UMAP plot showing distribution of PRDM1 − versus PRDM1 + T reg populations in HDs and patients with ATL. (G) Pie chart showing percentage of cells in HDs and patients with ATL that express any amount of PRDM1 . (H) Bar plot showing top pathways (by z score) <t>from</t> <t>Qiagen</t> <t>IPA</t> using differentially expressed genes derived from PRDM1 − versus PRDM1 + populations within T reg cells cluster in patients with ATL.
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Purified CD4 + T cells from HDs ( n = 3) and patients with acute ATL ( n = 5) were stimulated with anti-CD3 and anti-CD28 in presence of IL-2 for 4 hours at 37°C. The cells were harvested and subjected to scRNA-seq. ( A ) Schematic representation of the experimental setup for ( B to H ). Created in BioRender. S. Roy (2025) https://BioRender.com/9zdi679 . (B) Uniform Manifold Approximation and Projection (UMAP) plot showing all cells split by HD cells (left) and ATL cells (right). (C) Bar plot showing proportion of the cells in each cluster annotated by Azimuth for each HD and ATL sample. (D) UMAP plot showing distribution of T reg cells split by HD cells (left) and ATL cells (right). (E) Violin plot showing PRDM1 expression in T reg cells in HDs and patients with ATL. (F) UMAP plot showing distribution of PRDM1 − versus PRDM1 + T reg populations in HDs and patients with ATL. (G) Pie chart showing percentage of cells in HDs and patients with ATL that express any amount of PRDM1 . (H) Bar plot showing top pathways (by z score) <t>from</t> <t>Qiagen</t> <t>IPA</t> using differentially expressed genes derived from PRDM1 − versus PRDM1 + populations within T reg cells cluster in patients with ATL.
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Purified CD4 + T cells from HDs ( n = 3) and patients with acute ATL ( n = 5) were stimulated with anti-CD3 and anti-CD28 in presence of IL-2 for 4 hours at 37°C. The cells were harvested and subjected to scRNA-seq. ( A ) Schematic representation of the experimental setup for ( B to H ). Created in BioRender. S. Roy (2025) https://BioRender.com/9zdi679 . (B) Uniform Manifold Approximation and Projection (UMAP) plot showing all cells split by HD cells (left) and ATL cells (right). (C) Bar plot showing proportion of the cells in each cluster annotated by Azimuth for each HD and ATL sample. (D) UMAP plot showing distribution of T reg cells split by HD cells (left) and ATL cells (right). (E) Violin plot showing PRDM1 expression in T reg cells in HDs and patients with ATL. (F) UMAP plot showing distribution of PRDM1 − versus PRDM1 + T reg populations in HDs and patients with ATL. (G) Pie chart showing percentage of cells in HDs and patients with ATL that express any amount of PRDM1 . (H) Bar plot showing top pathways (by z score) <t>from</t> <t>Qiagen</t> <t>IPA</t> using differentially expressed genes derived from PRDM1 − versus PRDM1 + populations within T reg cells cluster in patients with ATL.
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Purified CD4 + T cells from HDs ( n = 3) and patients with acute ATL ( n = 5) were stimulated with anti-CD3 and anti-CD28 in presence of IL-2 for 4 hours at 37°C. The cells were harvested and subjected to scRNA-seq. ( A ) Schematic representation of the experimental setup for ( B to H ). Created in BioRender. S. Roy (2025) https://BioRender.com/9zdi679 . (B) Uniform Manifold Approximation and Projection (UMAP) plot showing all cells split by HD cells (left) and ATL cells (right). (C) Bar plot showing proportion of the cells in each cluster annotated by Azimuth for each HD and ATL sample. (D) UMAP plot showing distribution of T reg cells split by HD cells (left) and ATL cells (right). (E) Violin plot showing PRDM1 expression in T reg cells in HDs and patients with ATL. (F) UMAP plot showing distribution of PRDM1 − versus PRDM1 + T reg populations in HDs and patients with ATL. (G) Pie chart showing percentage of cells in HDs and patients with ATL that express any amount of PRDM1 . (H) Bar plot showing top pathways (by z score) from Qiagen IPA using differentially expressed genes derived from PRDM1 − versus PRDM1 + populations within T reg cells cluster in patients with ATL.

Journal: Science Advances

Article Title: BLIMP1 negatively regulates IL-2 signaling in T cells

doi: 10.1126/sciadv.adx8105

Figure Lengend Snippet: Purified CD4 + T cells from HDs ( n = 3) and patients with acute ATL ( n = 5) were stimulated with anti-CD3 and anti-CD28 in presence of IL-2 for 4 hours at 37°C. The cells were harvested and subjected to scRNA-seq. ( A ) Schematic representation of the experimental setup for ( B to H ). Created in BioRender. S. Roy (2025) https://BioRender.com/9zdi679 . (B) Uniform Manifold Approximation and Projection (UMAP) plot showing all cells split by HD cells (left) and ATL cells (right). (C) Bar plot showing proportion of the cells in each cluster annotated by Azimuth for each HD and ATL sample. (D) UMAP plot showing distribution of T reg cells split by HD cells (left) and ATL cells (right). (E) Violin plot showing PRDM1 expression in T reg cells in HDs and patients with ATL. (F) UMAP plot showing distribution of PRDM1 − versus PRDM1 + T reg populations in HDs and patients with ATL. (G) Pie chart showing percentage of cells in HDs and patients with ATL that express any amount of PRDM1 . (H) Bar plot showing top pathways (by z score) from Qiagen IPA using differentially expressed genes derived from PRDM1 − versus PRDM1 + populations within T reg cells cluster in patients with ATL.

Article Snippet: Pathway enrichment analyses were conducted using Qiagen IPA software (QIAGEN Inc., https://digitalinsights.qiagen.com/IPA ) version 107193442 ( ).

Techniques: Purification, Expressing, Derivative Assay